Extended Data

• Description: Figure 2 A: Floating mat collected from Onion Creek. B, C, and D: Micrographs of algal blooms collected in Austin creeks. Micrographs were photographed at 100X. These micrographs were taken from samples collected at Bull Creek (B, C), and Red Bud Isle (D). E: Sample sites for algal mat and water collection. Map created with Google Earth mapping software. F: Graphical summary of water quality variables. Median and interquartile range (IQR) was taken for the environmental factors measured for each sample site during the period of July to November 2024. Variables listed are nitrates (NO3 in ppm), phosphates (PO4 in ppm), temperature (℃), dissolved oxygen (DO in ppm), and conductivity (μS/cm). G: LC-MS data showing positive identification of cylindrospermopsin cyanotoxin at Onion Creek 10/11/2024. H: The relevant signal from a multi-reference cyanobacteria standard matches well to our sample peak (G). The x-axis shows retention time and the y-axis shows relative signal intensity. I: LC-MS data showing positive identification of dhATX in Red Bud Isle 12/2/2024. J: The relevant signal from a multi-reference cyanobacteria standard matches well with our sample peak (I). The x-axis shows retention time and the y-axis shows relative signal intensity.. Resource Type: Image. DOI: 10.22002/09ea9-n1173

Author Contributions

• Hanan Brower: Conceptualization, Investigation, Formal analysis, Methodology, Project administration, Validation, Visualization, Writing - original draft, Writing - review & editing
• Sahar Mahmood: Investigation, Methodology, Project administration, Formal analysis, Validation, Visualization, Writing - review & editing
• Joana Ruiz-Escobar: Investigation, Methodology, Project administration, Validation, Writing - review & editing
• Sara Bagheri: Investigation, Writing - review & editing
• Omar Carrasco-Rubio: Investigation, Writing - review & editing
• Luke Diggins: Investigation, Writing - review & editing
• Anastasia Kuzmina: Investigation, Writing - review & editing
• Wesley Tran: Investigation, Writing - review & editing
• Kevin Zhu: Investigation, Writing - review & editing
• Stuart Reichler: Funding acquisition, Investigation, Project administration, Supervision, Writing - review & editing, Resources, Visualization

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