microPublication Biology 2578-9430 Caltech Library 10.17912/micropub.biology.002347 new finding gene model drosophila Gene model for the ortholog of snu in Drosophila funebris McAlpin Braiden S. Data curation Formal analysis Investigation Writing - review & editing 1 Chialvo Pablo Investigation Formal analysis Validation Writing - original draft 1 Scott Chialvo Clare Conceptualization Supervision Validation Writing - review & editing 1 § Biology, Appalachian State University, Boone, North Carolina USA Turner Brandon Aumont Cedric Correspondence to: Clare Scott Chialvo ( chialvoch@appstate.edu )

The authors declare that there are no conflicts of interest present.

 29 9 2026 2026 2026 10.17912/micropub.biology.002347 12 8 2026 21 9 2026 29 9 2026 Copyright: © 2026 by the authors 2026 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

We developed a gene model for the ortholog of the Drosophila melanogaster gene snustorr ( snu ) in the ASM1890182v1 Genome Assembly (GenBank Accession: GCA_018901825.1 ) of Drosophila funebris . This ortholog was characterized as part of a developing dataset for a comparative study of detoxification gene family evolution in the immigrans - tripunctata radiation of the genus Drosophila using an adapted Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.

This gene annotation project was funded by Nation Science Foundation grants DEB-1737869 (PI LKR, CoPI CSC) and DBI-2217912 (PI CSC). The Genomics Education Partnership (GEP; https://thegep.org/ ), which supports this project, is funded by the National Science Foundation (1915544; PI LKR) and the National Institute of General Medical Sciences of the National Institutes of Health (R25GM130517; PI LKR). Any opinions, findings, and conclusions or recommendations expressed in this material are solely those of the author(s) and do not necessarily reflect the official views of the National Science Foundation nor the National Institutes of Health.

(A) Synteny comparison of the genomic neighborhoods for snu in Drosophila melanogaster and Drosophila funebris . Thin underlying arrows indicate which DNA strand the target gene, snu, is located on in D. melanogaster (top) and D. funebris (bottom). The thin arrow pointing to the right indicates that snu is on the positive strand in D. melanogaster , and the thin arrow pointing to the left indicates that snu is on the negative strand in D. funebris . The wide gene arrows pointing in the same direction as snu are on the same strand relative to the thin underlying arrows, while wide gene arrows pointing in the opposite direction of snu are on the opposite strand relative to the thin underlying arrows. White gene arrows in D. funebris indicate orthology to the corresponding gene in D. melanogaster , while black gene arrows indicate non-orthology. Gene symbols given in the D. funebris gene arrows indicate the orthologous gene in D. melanogaster , while the locus identifiers are specific to D. funebris . (B) Gene Model in GEP UCSC Track Data Hub (Raney et al., 2014). The coding-regions of snu in D. funebris are displayed in the User Supplied Track (red); coding sequences (CDS) are depicted by thick rectangles and introns by thin lines with arrows indicating the direction of transcription. Subsequent evidence tracks include Spaln of D. melanogaster Proteins (purple, alignment of Ref-Seq proteins from D. melanogaster ), Coding Regions Predicted by Augustus (dark blue), GeMoMa (teal), and NSCAN PASA-EST (dark green), and RNA-Seq from mixed sex adult flies (brown; alignment of Illumina RNA-Seq reads from D. funebris – Erlenbach et al., 2023). (C) Dot Plot of snu-PA in D. melanogaster ( x -axis) vs. the orthologous peptide in D. funebris ( y -axis). Amino acid number is indicated along the left and bottom; CDS number is indicated along the top and right, and CDSs are also highlighted with alternating colors. Line breaks in the dot plot indicate areas of with low sequence identity between species.

Description

Introduction

This article reports a predicted gene model generated by undergraduate work using a structured gene model annotation protocol defined by the Genomics Education Partnership (GEP; thegep.org) for Course-based Undergraduate Research Experience (CURE). The following information in quotes may be repeated in other articles submitted by participants using the same GEP CURE protocol for annotating Drosophila species orthologs of Drosophila melanogaster detoxification genes.

“Within insects, detoxifying xenobiotics and host secondary metabolites is a three-phase process that involves functionalization, conjugation, and excretion of these compounds. Expansions of known detoxification gene families ( e.g. , cytochrome P450s) are associated with diet breadth and insecticide resistance (Ranson et al., 2002; Després et al., 2007; Rane et al., 2016). With the increasing availability of high-quality genomes for non-model organisms, including Drosophila species beyond D. melanogaster , it is now possible to perform large scale comparative studies (Robinson et al., 2011; Kim et al., 2021; Threfall and Baxter, 2021). Careful manual annotation and curation of gene models can improve upon computational gene predictions in non-model species, which aids the accuracy of studies on gene and genome evolution (Mudge and Harrow, 2016; Tello-Ruiz et al., 2019). To aid in these annotations, the Genomics Education Partnership (thegep.org) developed a curriculum involving web-based tools that allow undergraduates to engage in authentic course-based research focused on manually annotating genes in non-model species (Rele et al., 2023). The orthologous gene models, including the one presented here, then provide a reliable basis for further evolutionary genomic analyses when made available to the scientific community. The gene ortholog described here in D. funebris for snustorr ( snu ), a member of the ABC transporter gene family, was characterized as part of a developing dataset for a comparative study of detoxification gene families in the immigrans - tripunctata radiation of the genus Drosophila .” (Williams et al., 2026)

“ Drosophila funebris (Fabricius, 1787) is a member of the funebris species group, which occurs in the immigrans-tripunctata radiation of the Drosophila subgenus (Bächli, 2005; ICZN, 2010). It is also the type species of the genus Drosophila . This species is a globally distributed human commensal (Grimaldi, 2022). While D. funebris feeds on shelf fungi and fruit (Kimura et al., 1977; Prigent et al., 2003), it does not tolerate the mushroom toxin α-amanitin (Stump et al., 2011; Erlenbach et al., 2023).” (McDonald et al., 2026).

"ATP-binding cassette (ABC) transporters constitute one of the largest protein superfamilies across all domains of life, functioning as primary-active transporters that use the energy of ATP hydrolysis to move a broad range of substrates across lipid membranes (Higgins, 1992; Dean et al., 2001). In the context of xenobiotic metabolism, ABC transporters mediate phase III detoxification by excreting toxins along with phase I and phase II metabolites out of cells. Their overexpression is a recurrent mechanism of insecticide and multidrug resistance in insects (Merzendorfer, 2014; Dermauw and Van Leeuwen, 2014)." (Dieterle et al., 2026)

snustorr ( snu ) is a member of the ABCH ATP-binding cassette transporter subfamily of ABC transporters. It is required for cuticle development and localizes to the apical plasma membrane and cytoplasmic vesicles, consistent with a role in exporting lipids to build the epidermal barrier (Zuber et al., 2018; Wang et al., 2020). Because the cuticular barrier is the insect's first defense against xenobiotic penetration, snu is considered part of the "Phase 0" detoxification program (Gao et al., 2022). Transcripts are enriched in the embryonic tracheal system and epidermis (Öztürk-Çolak et al., 2024).

We propose a gene model for the D. funebris ortholog of the D. melanogaster snustorr ( snu ) gene. The genomic region of the ortholog corresponds to the GeMoMa FBtr0085308_R0 gene prediction in the ASM1890182v1 Genome Assembly of D. funebris ( GCA_018901825.1 – Kim et al, 2021). This model is based on mixed sex, adult RNA-Seq data from D. funebris (Erlenbach et al., 2023; https://doi.org/10.5061/dryad.hdr7sqvq2) and snu in D. melanogaster using FlyBase release FB2024_02 ( GCA_000001215.4 ; Öztürk-Çolak et al., 2024).

Synteny

The reference gene, snu, occurs on chromosome 3R in D. melanogaster and is flanked upstream by huntingtin ( htt ) and Allatostatin A receptor 2 ( AstA-R2 ) and downstream by Stress induced DNase ( Sid ) and CG33346 . The tblastn search of D. melanogaster snu-PA (query) against the D. funebris (GenBank Accession: GCA_018901825.1 ) Genome Assembly (subject) placed the putative ortholog of snu within contig_683 ( JAEIFK010000678 ) which corresponds to GeMoMa gene prediction FBtr0085308_R0 (E-value: 0; percent identity: 92.53% as determined by blastp ). The putative ortholog is flanked downstream by FBtr0083949_R0, FBtr0083907_R0, and FBtr0083950_R0 (nested) which correspond to mitochondrial transcription factor A ( TFAM ), CG5191 , and CG10877 in D. melanogaster (E-value: 7e-142, 0, 0; identity: 69.42%, 96.97%, 70.79% respectively, as determined by blastp ; Figure 1A ; Altschul et al., 1990). The putative ortholog of snu is flanked upstream by FBtr0085310_R0 and FBtr0085316_R0, which correspond to htt and AstA-R2 in D. melanogaster (E-value: 0 and 6e-179; identity:66.34% and 70.95%, respectively, as determined by blastp ). The putative ortholog assignment for snu in D. funebris is supported by the following evidence: the tblastn results are of good quality, and all coding sequences (CDS) and isoforms found in D. melanogaster also appear to be present in D. funebris. Though the gene predictions immediately downstream of the putative snu ortholog are not conserved, they do appear in the same order and orientation much further downstream in D. melanogaster . Conversely, the upstream genetic neighborhood of the putative ortholog is conserved. As such, we conclude that FBtr0085308_R0 is an ortholog of snu in D. funebris ( Figure 1A ).

Protein Model

snu in D. funebris has ten CDS within the genomic sequence. All six isoforms (PA-PE, Figure 1B ) are translated from unique messenger RNAs. Relative to the ortholog in D. melanogaster , the CDS number and protein isoform count are conserved. The sequence of snu-PA in D. funebris has 92.5% identity (96.1% similarity) with the protein-coding isoform snu-PA in D. melanogaster , as determined by blastp ( Figure 1C ). Coordinates of this curated gene model are archived in the CaltechDATA repository (see “Extended Data” section below).

Methods

The annotation methods used in this project are adapted from those described in Rele et al. (2023), which includes algorithms, database versions, and citations for the complete annotation process developed for the Pathways Project. The methods for the current project are detailed in brief below with notes on significant differences between this protocol and the one described in Rele et al. (2023). The students use the GEP instance of the UCSC Genome Browser v.435 (https://gander.wustl.edu ; Kent et al., 2002; Raney et al., 2024) to examine the genomic neighborhood of their reference detoxification gene in the D. melanogaster genome assembly (Aug. 2014; BDGP Release 6 + ISO1 MT/dm6). Students obtain the protein sequence for the D. melanogaster target gene for a given isoform and use a tblastn search of the sequence against their target Drosophila species genome assembly ( D. funebris ( GCA_018901825.1 – Kim et al, 2021)) on the NCBI BLAST server (https://blast.ncbi.nlm.nih.gov/Blast.cgi, Altschul et al., 1990) to identify the putative ortholog location. Students compare the genomic neighborhood of the putative ortholog to that of the reference gene in D. melanogaster . This local synteny analysis includes a minimum of two upstream and two downstream genes relative to the potential ortholog. As no RefSeq protein data is available for these species, comparisons are based on gene predictions that correlate with gene expression data in the putative ortholog neighborhood. Using the multiple alignment tracks feature in the Genome Browser, students examine other sets of genomic evidence, including Spaln alignment of D. melanogaster proteins, multiple gene prediction tracks (e.g., GeMoMa, Augustus, NSCAN PASA-EST), and mixed sex RNA-Seq adult expression data from the target species generated by Erlenbach et al. (2023; https://doi.org/10.5061/dryad.hdr7sqvq2). Information on the genomic structure information (e.g., CDSs, intron-exon number, number of isoforms) for the reference gene in D. melanogaster is retrieved using Gene Record Finder (https://gander.wustl.edu/~wilson/dmelgenerecord/index.html; Rele et al ., 2023). To determine approximate splice sites within the target gene, a tblastn search using the CDSs from the D. melanogaste r reference gene against the putative ortholog location (10kb up- and downstream of the target gene prediction). Coordinates of the CDS(s) are refined by examining aligned RNA-Seq data, identifying canonical splice site sequences, and ensuring the maintenance of an open reading frame. Students confirm the biological validity of their target gene model using the FlySeq Gene Model Checker (https://gander2.wustl.edu/~wilson/genechecker-flyseq/), which compares the hypothesized target gene model's structure and translated sequence against the D. melanogaster reference gene. At least two independent models for this gene are generated. These models are reconciled by a third independent researcher to produce the final model presented here. Note: comparison of 5' and 3' UTR sequence information is not included in this GEP CURE protocol.

Extended Data

Description: Zipped archive containing FASTA, PEP, and GFF of snu model in D. funebris. Resource Type: Model. DOI: https://doi.org/10.22002/tqrd0-6z073

We would like to thank Wilson Leung for developing and maintaining the technological infrastructure that was used to create this gene model and Laura K. Reed for overseeing the Genomics Education Partnership. Thank you to FlyBase for providing the definitive database for Drosophila melanogaster gene models.

Altschul SF Gish W Miller W Myers EW Lipman DJ 1990 10 5 Basic local alignment search tool. J Mol Biol 215 3 0022-2836 403 410 10.1016/S0022-2836(05)80360-2 2231712 Bächli, G. (2005) Taxodros: The database on taxonomy of Drosophilidae, version February 2026, last accessed 28 May 2026. https://taxodros.uzh.ch/ Dean M Rzhetsky A Allikmets R 2001 7 1 The human ATP-binding cassette (ABC) transporter superfamily. Genome Res 11 7 1088-9051 1156 1166 10.1101/gr.184901 11435397 Dermauw W Van Leeuwen T 2013 11 28 The ABC gene family in arthropods: comparative genomics and role in insecticide transport and resistance. Insect Biochem Mol Biol 45 0965-1748 89 110 10.1016/j.ibmb.2013.11.001 24291285 Després L David JP Gallet C 2007 2 26 The evolutionary ecology of insect resistance to plant chemicals. Trends Ecol Evol 22 6 0169-5347 298 307 10.1016/j.tree.2007.02.010 17324485 Dieterle Summer Chialvo Pablo Scott Chialvo Clare 2026 Gene model for the ortholog of ABCB7 in Drosophila funebris 10.17912/micropub.biology.002346 Drosophila 12 Genomes Consortium. Clark AG Eisen MB Smith DR Bergman CM Oliver B Markow TA Kaufman TC Kellis M Gelbart W Iyer VN Pollard DA Sackton TB Larracuente AM Singh ND Abad JP Abt DN Adryan B Aguade M Akashi H Anderson WW Aquadro CF Ardell DH Arguello R Artieri CG Barbash DA Barker D Barsanti P Batterham P Batzoglou S Begun D Bhutkar A Blanco E Bosak SA Bradley RK Brand AD Brent MR Brooks AN Brown RH Butlin RK Caggese C Calvi BR Bernardo de Carvalho A Caspi A Castrezana S Celniker SE Chang JL Chapple C Chatterji S Chinwalla A Civetta A Clifton SW Comeron JM Costello JC Coyne JA Daub J David RG Delcher AL Delehaunty K Do CB Ebling H Edwards K Eickbush T Evans JD Filipski A Findeiss S Freyhult E Fulton L Fulton R Garcia AC Gardiner A Garfield DA Garvin BE Gibson G Gilbert D Gnerre S Godfrey J Good R Gotea V Gravely B Greenberg AJ Griffiths-Jones S Gross S Guigo R Gustafson EA Haerty W Hahn MW Halligan DL Halpern AL Halter GM Han MV Heger A Hillier L Hinrichs AS Holmes I Hoskins RA Hubisz MJ Hultmark D Huntley MA Jaffe DB Jagadeeshan S Jeck WR Johnson J Jones CD Jordan WC Karpen GH Kataoka E Keightley PD Kheradpour P Kirkness EF Koerich LB Kristiansen K Kudrna D Kulathinal RJ Kumar S Kwok R Lander E Langley CH Lapoint R Lazzaro BP Lee SJ Levesque L Li R Lin CF Lin MF Lindblad-Toh K Llopart A Long M Low L Lozovsky E Lu J Luo M Machado CA Makalowski W Marzo M Matsuda M Matzkin L McAllister B McBride CS McKernan B McKernan K Mendez-Lago M Minx P Mollenhauer MU Montooth K Mount SM Mu X Myers E Negre B Newfeld S Nielsen R Noor MA O'Grady P Pachter L Papaceit M Parisi MJ Parisi M Parts L Pedersen JS Pesole G Phillippy AM Ponting CP Pop M Porcelli D Powell JR Prohaska S Pruitt K Puig M Quesneville H Ram KR Rand D Rasmussen MD Reed LK Reenan R Reily A Remington KA Rieger TT Ritchie MG Robin C Rogers YH Rohde C Rozas J Rubenfield MJ Ruiz A Russo S Salzberg SL Sanchez-Gracia A Saranga DJ Sato H Schaeffer SW Schatz MC Schlenke T Schwartz R Segarra C Singh RS Sirot L Sirota M Sisneros NB Smith CD Smith TF Spieth J Stage DE Stark A Stephan W Strausberg RL Strempel S Sturgill D Sutton G Sutton GG Tao W Teichmann S Tobari YN Tomimura Y Tsolas JM Valente VL Venter E Venter JC Vicario S Vieira FG Vilella AJ Villasante A Walenz B Wang J Wasserman M Watts T Wilson D Wilson RK Wing RA Wolfner MF Wong A Wong GK Wu CI Wu G Yamamoto D Yang HP Yang SP Yorke JA Yoshida K Zdobnov E Zhang P Zhang Y Zimin AV Baldwin J Abdouelleil A Abdulkadir J Abebe A Abera B Abreu J Acer SC Aftuck L Alexander A An P Anderson E Anderson S Arachi H Azer M Bachantsang P Barry A Bayul T Berlin A Bessette D Bloom T Blye J Boguslavskiy L Bonnet C Boukhgalter B Bourzgui I Brown A Cahill P Channer S Cheshatsang Y Chuda L Citroen M Collymore A Cooke P Costello M D'Aco K Daza R De Haan G DeGray S DeMaso C Dhargay N Dooley K Dooley E Doricent M Dorje P Dorjee K Dupes A Elong R Falk J Farina A Faro S Ferguson D Fisher S Foley CD Franke A Friedrich D Gadbois L Gearin G Gearin CR Giannoukos G Goode T Graham J Grandbois E Grewal S Gyaltsen K Hafez N Hagos B Hall J Henson C Hollinger A Honan T Huard MD Hughes L Hurhula B Husby ME Kamat A Kanga B Kashin S Khazanovich D Kisner P Lance K Lara M Lee W Lennon N Letendre F LeVine R Lipovsky A Liu X Liu J Liu S Lokyitsang T Lokyitsang Y Lubonja R Lui A MacDonald P Magnisalis V Maru K Matthews C McCusker W McDonough S Mehta T Meldrim J Meneus L Mihai O Mihalev A Mihova T Mittelman R Mlenga V Montmayeur A Mulrain L Navidi A Naylor J Negash T Nguyen T Nguyen N Nicol R Norbu C Norbu N Novod N O'Neill B Osman S Markiewicz E Oyono OL Patti C Phunkhang P Pierre F Priest M Raghuraman S Rege F Reyes R Rise C Rogov P Ross K Ryan E Settipalli S Shea T Sherpa N Shi L Shih D Sparrow T Spaulding J Stalker J Stange-Thomann N Stavropoulos S Stone C Strader C Tesfaye S Thomson T Thoulutsang Y Thoulutsang D Topham K Topping I Tsamla T Vassiliev H Vo A Wangchuk T Wangdi T Weiand M Wilkinson J Wilson A Yadav S Young G Yu Q Zembek L Zhong D Zimmer A Zwirko Z Jaffe DB Alvarez P Brockman W Butler J Chin C Gnerre S Grabherr M Kleber M Mauceli E MacCallum I 2007 11 8 Evolution of genes and genomes on the Drosophila phylogeny. Nature 450 7167 0028-0836 203 218 10.1038/nature06341 17994087 Erlenbach T Haynes L Fish O Beveridge J Giambrone SA Reed LK Dyer KA Scott Chialvo CH 2023 12 13 Investigating the phylogenetic history of toxin tolerance in mushroom-feeding Drosophila. Ecol Evol 13 12 2045-7758 e10736 e10736 10.1002/ece3.10736 38099137 Fabricius Johann Christian Metcalf Collection (North Carolina State University). Tippmann Collection (North Carolina State University). 1787 Mantissa insectorum sistens eorum species nuper detectas adiectis characteribus genericis, differentiis specificis, emendationibus, observationibus, Ioh. Christ. Fabricii. 10.5962/bhl.title.36471 Gao L Zang X Qiao H Moussian B Wang Y 2022 8 8 Xenobiotic responses of Drosophila melanogaster to insecticides with different modes of action and entry. Arch Insect Biochem Physiol 111 4 0739-4462 e21958 e21958 10.1002/arch.21958 35942563 Grimaldi David A. 2022 4 7 The Drosophila funebris Species Group in North America (Diptera: Drosophilidae) American Museum Novitates 2022 3988 0003-0082 10.1206/3988.1 Higgins CF 1992 ABC transporters: from microorganisms to man. Annu Rev Cell Biol 8 0743-4634 67 113 10.1146/annurev.cb.08.110192.000435 1282354 ICZN. (2010) Opinion 2245 (Case 3407): Drosophila Fallén, 1823 (Insecta, Diptera): Drosophila funebris Fabricius, 1787 is maintained as the type species. The Bulletin of Zoological Nomenclature 67(1), 106-115. Kent WJ Sugnet CW Furey TS Roskin KM Pringle TH Zahler AM Haussler D 2002 6 1 The human genome browser at UCSC. Genome Res 12 6 1088-9051 996 1006 10.1101/gr.229102 12045153 Kim BY Wang JR Miller DE Barmina O Delaney E Thompson A Comeault AA Peede D D'Agostino ERR Pelaez J Aguilar JM Haji D Matsunaga T Armstrong EE Zych M Ogawa Y Stamenković-Radak M Jelić M Veselinović MS Tanasković M Erić P Gao JJ Katoh TK Toda MJ Watabe H Watada M Davis JS Moyle LC Manoli G Bertolini E Košťál V Hawley RS Takahashi A Jones CD Price DK Whiteman N Kopp A Matute DR Petrov DA 2021 7 19 Highly contiguous assemblies of 101 drosophilid genomes. Elife 10 10.7554/eLife.66405 34279216 Kimura M, Toda M, Beppu K, Watabe H. (1977) Breeding sites of Drosophilid flies in and near Sapporo northern Japan, with supplementary notes on adult feeding habits. Japanese Journal of Entomology 45: 571-582. McDonald M, Chialvo P, Scott Chialvo C. 2026. Gene model for the ortholog of PGRP-SB1 in Drosophila funebris . microPublication Biology. 10.17912/micropub.biology.002270. Merzendorfer Hans 2014 ABC Transporters and Their Role in Protecting Insects from Pesticides and Their Metabolites Advances in Insect Physiology 0065-2806 1 72 10.1016/b978-0-12-417010-0.00001-x Mudge JM Harrow J 2016 10 24 The state of play in higher eukaryote gene annotation. Nat Rev Genet 17 12 1471-0056 758 772 10.1038/nrg.2016.119 27773922 Öztürk-Çolak A Marygold SJ Antonazzo G Attrill H Goutte-Gattat D Jenkins VK Matthews BB Millburn G Dos Santos G Tabone CJ FlyBase Consortium 2024 2 1 FlyBase: updates to the Drosophila genes and genomes database. Genetics 0016-6731 10.1093/genetics/iyad211 38301657 Prigent Stéphane Renard Emmanuelle Cariou Marie-Louise 2003 10 1 Electrophoretic Mobility of Amylase in Drosophilids Indicates Adaptation to Ecological Diversity Genetica 119 2 0016-6707 133 145 10.1023/a:1026071317995 Rane RV Walsh TK Pearce SL Jermiin LS Gordon KH Richards S Oakeshott JG 2015 12 29 Are feeding preferences and insecticide resistance associated with the size of detoxifying enzyme families in insect herbivores? Curr Opin Insect Sci 13 70 76 10.1016/j.cois.2015.12.001 27436555 Raney BJ Barber GP Benet-Pagès A Casper J Clawson H Cline MS Diekhans M Fischer C Navarro Gonzalez J Hickey G Hinrichs AS Kuhn RM Lee BT Lee CM Le Mercier P Miga KH Nassar LR Nejad P Paten B Perez G Schmelter D Speir ML Wick BD Zweig AS Haussler D Kent WJ Haeussler M 2024 1 5 The UCSC Genome Browser database: 2024 update. Nucleic Acids Res 52 D1 0305-1048 D1082 D1088 10.1093/nar/gkad987 37953330 Raney BJ Dreszer TR Barber GP Clawson H Fujita PA Wang T Nguyen N Paten B Zweig AS Karolchik D Kent WJ 2013 11 13 Track data hubs enable visualization of user-defined genome-wide annotations on the UCSC Genome Browser. Bioinformatics 30 7 1367-4803 1003 1005 10.1093/bioinformatics/btt637 24227676 Ranson H Claudianos C Ortelli F Abgrall C Hemingway J Sharakhova MV Unger MF Collins FH Feyereisen R 2002 10 4 Evolution of supergene families associated with insecticide resistance. Science 298 5591 0036-8075 179 181 10.1126/science.1076781 12364796 Rele CP Sandlin KM Leung W Reed LK 2023 10 13 Manual annotation of Drosophila genes: a Genomics Education Partnership protocol. F1000Res 11 1579 1579 10.12688/f1000research.126839.3 37854289 Robinson GE Hackett KJ Purcell-Miramontes M Brown SJ Evans JD Goldsmith MR Lawson D Okamuro J Robertson HM Schneider DJ 2011 3 18 Creating a buzz about insect genomes. Science 331 6023 0036-8075 1386 1386 10.1126/science.331.6023.1386 21415334 Stump AD Jablonski SE Bouton L Wilder JA 2011 12 1 Distribution and mechanism of α-amanitin tolerance in mycophagous Drosophila (Diptera: Drosophilidae). Environ Entomol 40 6 0046-225X 1604 1612 10.1603/EN11136 22217779 Tello-Ruiz MK Marco CF Hsu FM Khangura RS Qiao P Sapkota S Stitzer MC Wasikowski R Wu H Zhan J Chougule K Barone LC Ghiban C Muna D Olson AC Wang L Ware D Micklos DA 2019 10 28 Double triage to identify poorly annotated genes in maize: The missing link in community curation. PLoS One 14 10 e0224086 e0224086 10.1371/journal.pone.0224086 31658277 Threlfall J Blaxter M 2021 5 21 Launching the Tree of Life Gateway. Wellcome Open Res 6 2398-502X 125 125 10.12688/wellcomeopenres.16913.1 34095514 Wang Y Norum M Oehl K Yang Y Zuber R Yang J Farine JP Gehring N Flötenmeyer M Ferveur JF Moussian B 2020 1 13 Dysfunction of Oskyddad causes Harlequin-type ichthyosis-like defects in Drosophila melanogaster. PLoS Genet 16 1 1553-7390 e1008363 e1008363 10.1371/journal.pgen.1008363 31929524 Williams E Chialvo P Scott Chialvo C 2026 5 27 Gene model for the ortholog of GstO3 in Drosophila dunni. MicroPubl Biol 2026 10.17912/micropub.biology.002110 42294398 Zuber R Norum M Wang Y Oehl K Gehring N Accardi D Bartozsewski S Berger J Flötenmeyer M Moussian B 2017 12 27 The ABC transporter Snu and the extracellular protein Snsl cooperate in the formation of the lipid-based inward and outward barrier in the skin of Drosophila. Eur J Cell Biol 97 2 0171-9335 90 101 10.1016/j.ejcb.2017.12.003 29306642